Fig. 8.

NEAT1 knockdown represses LD agglomeration and inhibits autophagy following MCAO in vivo. Mice were exposed to 45 min of MCAO followed by 7 days of survival. Sham group mice underwent the surgery procedure without MCAO. a Images show the dual fluorescence staining for BODIPY and CD11b in the ischemic hemisphere taken from the sham group, the negative control group (MCAO + ASO scramble) or mice treated with ASO NEAT1 at 7 dpi. Quantification of the mean BODIPY⁺ fluorescence intensity expressions between each group of mice is shown in b. c, d Western blot analysis of PLIN2 in whole ipsilateral cortex tissues and β-actin was used as a reference. e–f The expression of PLIN2 and TREM2 in the ipsilateral ischemic cerebral cortex from sham-operated mice (sham + ASO scramble, sham + ASO NEAT1) or from mice at 7 dpi (MCAO + ASO scramble, MCAO + ASO NEAT1) was examined by qRT-PCR. g–j Representative images of western blots are shown for the expression of p62 (g) and LC3 (i) in whole ipsilateral cortex tissues. Bar graphs show the quantitative analyses of western blots as ratios of p62/β-actin (h) and LC3/β-actin (j). All results are expressed as mean ± standard deviation and analyzed by one-way ANOVA (n = 4 mice per experimental group) followed by Tukey's post-hoc-test. NS, no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Abbreviations: LD, lipid droplets; ASO, antisense oligonucleotide; MCAO, middle cerebral artery occlusion; PLIN2, perilipin 2; TREM2, triggering receptor expressed on myeloid cells 2; NEAT1, nuclear paraspeckle assembly transcript 1; dpi, day post-ischemia