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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Adv Biol (Weinh). 2023 Jul 12;7(12):e2300157. doi: 10.1002/adbi.202300157

Figure 1. Detection of CD82 expression in human adult skeletal muscle frozen tissue sections.

Figure 1.

(A-C) Detection of CD82 at the myofiber membrane of unfixed sections and localizing with dystrophin. CD82 localization at the myofiber membrane is labeled by the arrow, while presumed interstitial cells are marked by an open arrowhead. (D-F) Co-localization of CD82 with CD31 in unfixed tissue sections shows labeling of capillaries and endothelial cells (arrow). Additionally, cells negative for CD31 but positive for CD82 expression were detected (filled arrowhead). (G-I) PFA-fixed tissue sections to co-localize Pax7 and CD82. Co-immunostaining of CD82 (green) and Pax7 (red) shows that CD82 labels the membrane of satellite cells in human muscle tissue sections. The myofiber sarcolemma is also labeled by CD82, but less intensely. J-L. Images of frozen unfixed tissue sections processed in parallel, where the primary antibody was omitted. Images were acquired at high exposure (using autoexposure), to show lack of signal and confirm specificity of staining when using the primary antibodies. Scale bars are indicated in merged images.