Table 4.
Antibodies and dyes for immunofluorescence (IF) and western blot.
| Marker | Supplier | Catalog # | Host species | IF | Western blot |
|---|---|---|---|---|---|
| β-Catenin | Santa Cruz (Dallas, TX, USA) | sc-7963 | Mouse | 1:500 | 1:2000 |
| β-III Tubulin (TUJ1) | R&D Systems (Minneapolis, MN, USA) | MAB1195 | Mouse | 1:200 | |
| Claudin 1 | Cell Signaling Technologies (Danvers, MA, USA) | 13995 | Rabbit | 1:200 | 1:1000 |
| Cleaved Caspase 3 (Active) | BD Biosciences (Franklin Lakes, NJ, USA) | 559565 | Rabbit | 1:500 | |
| GAPDH | MilliporeSigma | MAB374 | Mouse | 1:5000 | |
| GFAP | Cell Signaling Technology | 3670 | Mouse | 1:300 | |
| Ki67 | Abcam (Cambridge, UK) | ab16667 | Rabbit | 1:250 | |
| MAP2 | MilliporeSigma | M9942 | Mouse | 1:500 | |
| N-Cadherin | BD Biosciences | 610921 | Mouse | 1:500 | 1:2000 |
| Nestin | ThermoFisher | 14-9843-82 | Mouse | 1:500 | |
| OCT4 BrilliantViolet™421 | BioLegend (San Diego, CA, USA) | 653712 | Mouse | 1:40 | |
| PANX1 | Laird Lab [99] | N/A | Rabbit | 1:500 | 1:2000 |
| PAX6 PerCP-Cy™5.5 | BD Biosciences | 562388 | Mouse | 1:100 | |
| Pericentrin | Abcam | ab4448 | Rabbit | 1:1000 | |
| SOX2 | R&D Systems | AF2018 | Goat | 1:200 | |
| ZO-1 | ThermoFisher | 33-910 | Mouse | 1:500 | |
| Hoechst 33342 | FisherScientific | H3570 | Dye | 1:1000 | |
| Phalloidin-555 | ThermoFisher | A34055 | Dye | 1:500 | |
| Phalloidin-647 | Cell Signaling Technology | 8940 | Dye | 1:20 | |
| To-Pro™−3 Iodide (642/661) | ThermoFisher | T3605 | Dye | 1:1000 |