FIG. 5.

Effects of supernatants from mannose-treated A. castellanii trophozoites on CHCE cell monolayers. Trophozoites were incubated with 100 mM mannose for 48 h at 35°C. Supernatants were collected, centrifuged, filter sterilized, concentrated fourfold, and dialyzed against a 10-kDa-cutoff membrane. The concentrated supernatants were added to CHCE cell monolayers and incubated at 35°C for 48 h (A). MEM containing 100 mM mannose, but not exposed to trophozoites, was similarly treated and added to monolayers (B). Monolayers were photographed 48 h later. Bar = 100 μm.