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Molecular weight estimation of Acanthamoeba-derived cytolytic factors. Trophozoites were incubated with 100 mM mannose for 48 h at 35°C. Supernatants were collected, centrifuged, filter sterilized, and size fractionated with centrifugal concentrators having a molecular mass cutoff of either 30 or 100 kDa. Fractions were added to CHCE cell monolayers, and CPE was assessed spectrophotometrically. Results are expressed as mean ± standard deviation.
