Immunoprecipitation analysis of P. falciparum proteins. Parasites were grown and labelled as previously described (21). Labelled polypeptides were subjected to immunoprecipitation with fraction 2 (lane 1, culture supernatant; lane 2, cell pellet), fraction 1 (lane 3, culture supernatant; lane 4, cell pellet), or purified IgG from Danish donors never exposed to malaria (lane 5, culture supernatant; lane 6, cell pellet). The sizes (in kilodaltons) of molecular mass markers are indicated on the right.