FIG. 4.

ADCI assay. (A) Shown are the means of the SGI (calculated as described in Materials and Methods) obtained with the following antibodies: fraction 1 (135 μg/ml) (number of independent experiments [n = 1) and fraction 2 (135 μg/ml) (n = 3); IgG (200 μg/ml) from normal French blood donors (n = 2), IgG (200 μg/ml) from hyperimmune African blood donors (n = 6), and a 1/10 solution in RPMI 1640 of the antibodies affinity purified on peptides GL5 (n = 3) and GL9 (n = 3) as described in Materials and Methods. Human affinity-purified antibodies on MSP-3b (n = 3) and RESA (n = 3), diluted 1/10 in RPMI 1640, were included as positive and negative controls, respectively (23). (B) Dose dependency of anti-GLURP antibodies in ADCI experiments. Shown is the SGI plotted versus the IgG concentration for fraction 1 (n = 3) and fraction 2 (n = 2). (C) Comparison of ADCI effects of the fractions 2 to 5. Shown are the means of the SGI of purified IgG adjusted to 45 μg/ml (fractions 2, 3, and 4) and 200 μg/ml (fraction 5 and normal French IgG). The experiments with fraction 2 and normal French IgG were performed twice.