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. 2024 Jan 12;29:12. doi: 10.1186/s11658-023-00530-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Profile of Circle-seq data for eccDNAs. A The volcano plot of differentially expressed eccDNA in the serum of newly diagnosed T2DM patients and NC subjects. B The top 42 upregulated circulating eccDNAs in T2DM patients compared with NC subjects. C Five upregulated eccDNAs were selected to validate using qPCR with the outward facing primers. D The 10 most enriched KEGG pathways in T2DM patients based on eccDNAs carrying genes