Fig. 4.
Expression of eccDNA SORBS1circle in HG/PA-treated HepG2 cells. A, B The protein levels of P-Akt S473 and T-AKT in HepG2 cells treated with 25.0 mM glucose (HG) and 0.5 mM palmitate (PA) were evaluated by western blotting. These HepG2 cells were treated with 100 nM insulin for 20 min before being harvested. C qPCR was performed for the determination of intracellular eccDNA SORBS1circle level in HG/PA-treated HepG2 cells. D Visualization of eccDNA SORBS1circle expression in control and HG/PA-treated HepG2 cells using FISH assay. The complete image of FISH assay was shown in Additional file 1: Fig. S1. Bar = 10 μm. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01