Fig. 5.

Source of eccDNA SORBS1^circle in HG/PA-treated HepG2 cells. A–D DNA fragmentation, Bcl-2 and Bax protein expression, and eccDNA SORBS1^circle level were determined in HepG2 cells stimulated with HG/PA for 36 h in the presence or absence of 100 μM Z-VAD-FMK. E Visualization of eccDNA SORBS1^circle expression in HepG2 cells stimulated with HG/PA for 36 h in the presence or absence of 100 μM Z-VAD-FMK using FISH assay. The complete image of FISH assay was shown in Additional file 1: Fig. S2. Bar = 10 μm. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01