Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Construction of fliC mutants demonstrated by Southern hybridization. A fliC probe consisting of a 659-bp EcoRI-SalI DNA fragment from pMS590 was used to detect homologous DNA cleaved with EcoRI and NruI in pMS590 (fliC interrupted by a Gm cassette) (lane A), 32P-λ HindIII-labeled molecular weight markers (lane B), PAK genomic digest (lane C), PAKfliC (lane D), and PAK/NPfliC (lane E).
