FIG. 3.

Binding of flagellin and pilin to GM1 and asialoGM1 quantified by ELISA. (A) Pilin binding to microtiter plates coated with either asialoGM1 (AGM1) or GM1, detected by using antipilin antisera and alkaline phosphatase-conjugated goat anti-rabbit antibody and plotted by the relative OD₄₀₅ determined in an ELISA reader. (B) Flagellin binding to asialoGM1 and GM1, similarly compared by using antiflagellin antisera and alkaline phosphatase-conjugated goat anti-rabbit antibody as measured by ELISA.