TABLE 1.
Effect of IL-12 neutralizing antibody on intrapulmonary replication of L. pneumophilaa
| Duration of infection (h) | Antibody titerb
|
|
|---|---|---|
| Untreated | Anti-IL-12 | |
| 24 | (6.00 ± 2.67) × 107 | (1.27 ± 4.68) × 108 |
| 72 | (1.17 ± 0.43) × 107 | (2.23 ± 0.63) × 107 |
| 120 | (4.53 ± 3.21) × 105 | (1.37 ± 0.38) × 107* |
a
A/J mice were administered IL-12 neutralizing antibody as described in Materials and Methods prior to intratracheal inoculation with L. pneumophila (106 bacteria per mouse). At selected time points thereafter, the mice were euthanized and growth of L. pneumophila in the lung was determined and compared to that of untreated mice (i.e., immunocompetent mice inoculated intratracheally with L. pneumophila).
b
Mean ± standard error of the mean for 8 to 14 animals per treatment group. *, significantly greater than value for control mice (Student’s t test, P < 0.05).