FIG. 1.

Construction of IpaC-C3 hybrid proteins. (A) C3 insertion sites (the restriction site used for cloning and the resulting position of C3 within the IpaC sequence are indicated by arrows), size of the insert, and modification of IpaC sequence resulting from genetic manipulation. (B) Amino acid residues and corresponding DNA sequence of the C3 epitope. (C) Amino acid residues and corresponding DNA sequences of IpaC-C3 hybrid proteins for each insertion site, indicated as boldface characters inside parentheses for C3, boldface characters outside parentheses for IpaC, and lightface characters for additional sequences resulting from cloning. IpaC residue numbers are indicated.