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Production of IpaC-C3 hybrid proteins in SC602. (A, B, and D) Detection of IpaC-C3 hybrid proteins in whole-cell extracts with the anti-IpaC K24 MAb (A) or anti-C3 polyclonal serum (B) and in culture supernatants with anti-C3 serum (D) by immunoblot analysis of exponentially grown cultures. (C) SDS-PAGE and Coomassie blue staining of whole-cell extracts of stationary-phase cultures after formaldehyde treatment. The arrowhead indicates the position of the hybrid proteins.
