a, Experimental procedure to measure plasmid loss.
b, c, Evaluation of change of total cell counts based on different testing conditions under the selection of kanamycin or kanamycin and chloramphenicol after 5 h of growth with/without induction. n=4 biologically independent samples. Bar graph represents mean ± SD.
d, Comparison of change of fraction of cells carrying both plasmids based on the location of the parS site after 5 h of induction with 0.5 mM IPTG. n=3 biologically independent samples. Bar graph represents mean ± SD.
e, Evaluation of the contribution of dimerization domain on modulating the fraction of cells carrying two plasmids after 90 min of induction with 0.5 mM IPTG. n=3 biologically independent samples. **, p=0.0079 by a two-tailed unpair t-test. Bar graph represents mean ± SD.