Fig. 3 |. Evaluations of the molecular dynamics and permeability of synthetic DNA condensates.

a, Condensates formed by 500 nM WT-dParB (DBD) (doped with 10% WT-DBD-sfGFP) with 10 nM DNA (cy3-labeled) containing 1 parS site. Scale bar is 10 µm. WT-DBD is labeled green (left panel). DNA is labeled red (middle panel).

b, Super-resolution images showing deconvoluted 3D construction of a single DNA condensate. WT-DBD is labeled green (left panel). DNA is labeled red (middle panel).

c, Condensates formed by 500 nM WT-DBD (doped with 10% RLP_WT-DBD-sfGFP) with 10 nM Cy3-labeled DNA containing 2 parS site. Scale bar is 10 µm.

d, Representative FRAP images and derived diffusion coefficients of 500 nM synIDP-DBD-DD in the DNA condensates (doped with 10% synIDP-DBD-DD-sfGFP). Scale bar is 5 µm. n = 3 independent experiments. **, p=0.0092 by two-tailed unpaired t-test.

e, Permeability of the DNA condensates formed by RLP_S-Y and RLP_WT -DBD-DD-DNA to fluorescein labeled dextran 40 kDa. Both RLP_S-Y and RLP_WT mediated DNA condensate exclude 40 kDa dextran with a partition coefficient smaller than 1. Condensates are shown in the bright-field images in the middle. Scale bar is 10 µm. n = 6 independent experiments.

f, Representative FRAP images and derived diffusion coefficients of 500 nM synIDP-DBD in the DNA condensates (doped with 10% synIDP-DBD-sfGFP). Scale bar is 5 µm. ns, non-significant, p=0.1173 by two-tailed unpaired t-test. n = 3 independent experiments.

g, Permeability of the condensates formed by RLP_WT and RLP_R-D2-DBD with DNA to Antonia red labeled dextran 150 kDa. Both RLP_WT and RLP_RD2 mediated DNA condensates are permeable to 150 kDa dextran with a partition coefficient larger than 1. Condensates are shown in the green fluorescence channel in the middle. Scale bar is 10 µm. n = 6 independent experiments.

h, Representative FRAP images and fraction of recovery of different condensates in living cells.

Scale bar is 5 µm. n = 3 independent experiments.

Bar graph represents mean± SD