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. Author manuscript; available in PMC: 2024 Jan 12.
Published in final edited form as: Water Res. 2022 Jan 22;212:118114. doi: 10.1016/j.watres.2022.118114

Table 2.

Summary of standard curve performance metrics based on six instrument runs for each qPCR assay using NIST SRM® 2917.

Assay Slope Y-Intercept E ± std R2 LLOQ
CowM2 −3.36 ± 0.01 39.4 ± 0.06 0.98 ± 0.006 0.999 36.0
CowM3 −3.40 ± 0.01 37.7 ± 0.07 0.97 ± 0.005 0.998 34.3
CPQ_056 −3.35 ± 0.02 38.6 ± 0.13 0.99 ± 0.008 0.996 35.4
CPQ_064 −3.45 ± 0.01 39.9 ± 0.05 0.95 ± 0.006 0.998 36.4
DG3 −3.38 ± 0.04 35.9 ± 0.14 0.98 ± 0.014 0.998 32.7
DG37 −3.36 ± 0.02 36.1 ± 0.07 0.98 ± 0.008 0.998 32.8
EC23S857 −3.37 ± 0.02 36.7 ± 0.07 0.98 ± 0.008 0.998 33.3
Entero1a −3.37 ± 0.01 36.3 ± 0.05 0.98 ± 0.006 0.998 32.9
GFD −3.36 ± 0.02 34.6 ± 0.07 0.98 ± 0.008 0.998 31.2
HF183/BacR287 −3.37 ± 0.02 36.2 ± 0.08 0.98 ± 0.009 0.997 32.8
HumM2 −3.34 ± 0.03 38.7 ± 0.11 0.99 ± 0.011 0.997 35.4
Pig2Bac −3.36 ± 0.03 35.7 ± 0.12 0.98 ± 0.011 0.996 32.4
Rum2Bac −3.44 ± 0.02 39.3 ± 0.07 0.95 ± 0.009 0.998 35.9

E ± std denotes amplification efficiency [10(−1/slope) −1] ± standard deviation.

R2 represents linearity of the fitted curve.

LLOQ indicates lower limit of quantification reported as the quantitative threshold (Cq).