A) Effect of compound treatment on cellular OSBP expression in HCT-116 and HEK293 cells. Cells were treated with DMSO vehicle control, OSW-1 (1 nM), Taxol (10 nM), ITZ (10,000 nM), TTP (10,000 nM) or THEV (10,000 nM) for 24 h. Representative Western blot and average of multiple independent experiments shown. B) OSBP levels via Western blot in HeLa cells treated with compound (OSW-1 10 nM, THEV 10,000 nM, ITZ 10,000 nM, or TTP 10,000 nM) for 6 h followed by cell washing and incubation in compound-free media for 24 h. Western blot represents OSBP protein levels at the time of inoculation during washout treatment in anti-viral experiments (see Figure 2).