A) Viability of cells using Trypan Blue stain after 24 h of compound treatment or co-administration of OSW-1 (1 nM) with 10,000 nM of ITZ, THEV, or TTP. B) Cytotoxicity of 1 nM OSW-1 at 48 h co-administered with 10,000 nM THEV, TTP, or ITZ in HCT-116 and HEK293 cells. Representative cytotoxicity curves and average GI50 of three independent experiments shown. C) OSBP levels in HCT-116 and HEK293 cells of co-administration of 1 nM of OSW-1 with 10,000 nM of ITZ, THEV or TTP for 24 h. Representative Western blot and average of three independent experiments shown.