A) Immunofluorescent confocal microscopy of HCT-116 cells treated for 24 h with either DMSO (vehicle), 10,000 nM 25-OHC, 1 nM OSW-1, 10,000 nM ITZ, 10,000 nM TTP, or 10,000 nM THEV. OSBP shown in green; Golgi marker TGN46 shown in red; nucleus stain in blue. The merged images indicates colocalization of the OSBP and Golgi marker. B) Co-administration of 1 nM OSW-1 with 10,000 nM ITZ, 10,000 nM TTP, or 10,000 nM THEV.