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. 2024 Jan 2;20(1):e1011914. doi: 10.1371/journal.ppat.1011914

Fig 5. CD4 T cells from RORγt mutant mice have a similar expression pattern of Th1 and Th17 markers as wild-type mice.

Fig 5

Lymphocytes isolated from the FRT and ILN were stimulated with PMA and ionomycin with Brefeldin A before staining for flow cytometry. Results are gated on CD4+ CD44hi cells. (A) Percentage and number of total activated CD4 T cells isolated from FRT and ILN. (B) Expression of Th1 markers T-bet and IFN-γ. (C) Summary graphs from B. (D) Flow cytometry of expression of Th17 markers RORγt and IL-17A. (E) Summary graphs from D. All graphs are displayed as mean ± SD. Data is representative of two experiments.