FIG. 5.

Differential effects of Brefeldin A on antigen processing of M. tuberculosis bacilli for γδ and CD4⁺ T cells. Monocytes incubated for 12 h with either M. tuberculosis (MTB) (A, B, and C) or cytosolic mycobacterial antigens (Ag) (D) were treated with Brefeldin A (BFA) for 120 min before serving as targets in a CTL assay with either γδ T-cell lines (A, C, and D) or CD4⁺ T cell lines (B). In experiment 1 (EXP 1), the same monocyte targets were used for both CD4⁺ and γδ T-cell lines, with the γδ T-cell lines being derived from an HLA-mismatched donor. Results are representative of four experiments. E:T ratio, effector-to-target ratio.