FIG. 6.

Effect of an inhibitor of lysosomal acidification on antigen processing of M. tuberculosis bacilli by monocytes for CD4⁺ T-cell lines. Monocytes incubated for 12 h with either M. tuberculosis (MTB) (A and B) or cytosolic antigens (Ag) (C and D) of M. tuberculosis were treated with chloroquine (Chloro) for 120 min before serving as targets in a CTL assay with CD4⁺ T cells. The long-term CD4⁺ T-cell line used in experiment 1 (EXP. 1) was generated against total cytosolic proteins of M. tuberculosis, and the long-term CD4⁺ T-cell line used in experiment 2 was generated against purified 30-kDa (85B) antigen of M. tuberculosis. Results are representative of six experiments. E:T ratio, effector-to-target ratio.