Fig 4. Binding of rTsTryp to IEC and its cellular localization by IFA.

A: Binding of rTsTryp and IECs was observed by fluorescence microscope (400×). B: Confocal microscopy showed that bind of rTsTryp and IEC was mainly located in cytomembrane and a little in cytoplasm. In the IFA test, the primary antibodies were T. spiralis-infected serum, anti-rTsTryp serum, normal serum and anti-TRX serum, respectively. Alexa Fluor 488-conjugated anti-mouse IgG was served as the secondary antibody. Nuclei were stained blue using DAPI. Scale bars: 5 μm