Table 2. The SagGDvasa GD shows high homing rates in the G2 generation at both the Lp and Saglin loci.
Six individual SagGDvasa G1 females mated to WT males oviposited in individual tubes and their larval progeny was scored visually for GFP and DsRed fluorescence. Homing rates are calculated as the percentage of WT chromosomes converted to transgenic, i.e.: ((inheritance rate)–50%)x2.
| Female # | Total larvae | Negatives | GFP + only | DsRed + only | GFP + DsRed + | GFP inheritance and homing rate(Lp locus) | DsRed inheritance rate, homing rate(Saglin locus) |
|---|---|---|---|---|---|---|---|
| 1 | 89 | 0 | 0 | 0 | 89 | 100 % | 100 %, 100% |
| 2 | 52 | 0 | 4 | 0 | 48 | 100 % | 92.3%, 84.6 % |
| 3 | 59 | 0 | 0 | 0 | 59 | 100 % | 100%, 100 % |
| 4 | 59 | 0 | 6 | 0 | 53 | 100 % | 89.8%, 79.6 % |
| 5 | 50 | 0 | 5 | 0 | 45 | 100 % | 90%, 80 % |
| 6 | 57 | 0 | 3 | 0 | 54 | 100 % | 94,7%, 89.4 % |