FIG. 5.

Binding of salivary components to rPAc. The binding to rPAc of high-molecular-mass glycoprotein and sIgA components separated from salivary agglutinin by electrophoretic fractionation, sIgA purified by using jacalin-agarose, and salivary lysozyme was determined by BIAcore assay. Values are given as the means ± standard deviations of RU in triplicate assays. Symbols: ○, salivary agglutinin; •, high-molecular-mass glycoprotein separated by electrophoretic fractionation; □, sIgA components separated by electrophoretic fractionation; ▪, sIgA purified by using jacalin-agarose; ▵, salivary lysozyme.