TABLE 3.
Effects of cations on the binding of salivary agglutinin to rPAca
| Cation | Binding of salivary agglutinin to rPAc at indicated concn (mM)b
|
|||
|---|---|---|---|---|
| 0 | 0.1 | 1.0 | 2.5 | |
| Calcium | 7 ± 1 | 242 ± 8 | 794 ± 17 | 726 ± 28 |
| Magnesium | 5 ± 1 | 6 ± 1 | 7 ± 0 | 10 ± 1 |
| Manganese | 6 ± 1 | 5 ± 1 | 5 ± 1 | 7 ± 1 |
a
Salivary agglutinin (25 μg/ml) in PBS (pH 7.0) containing various amounts of CaCl2, MgCl2, or MnCl2 was allowed to react with rPAc immobilized on a sensor chip.
b
Binding is expressed as RU determined by BIAcore assay. The values are the means ± standard deviations of triplicate assays.