Fig. 7.

pS273R promotes HSV-1 and VSV replication by inhibiting IFN-I production. A, B HeLa cells were transfected with empty or Myc-S273R plasmid. At 12 hpt, cells were infected with HSV-1 or VSV at an MOI of 0.001 for 24 ​h and then were analyzed by fluorescence microscope. Scale bar, 500 ​μm. C–F HeLa cells were transfected with empty or Myc-S273R plasmid for 12 ​h and then infected with HSV-1 (MOI ​= ​0.01) or VSV (MOI ​= ​0.01) for the indicated times. The infected cells and supernatants were harvested to analyze IFN-β expression (C, D). HSV-1 UL30 and VSV N gene transcriptions were measured by qRT-PCR (E). The viral titer in the supernatant from HSV-1 or VSV infected cells was analyzed by TCID₅₀ assay (F). Data are presented as mean ​± ​SD of three independent experiments. Statistical significance between groups was determined using a t-test with GraphPad Prism software. ∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001.