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. 2023 Dec 13;11:rbad108. doi: 10.1093/rb/rbad108

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Modification and characterization of Exo^CTP. (A) Schematic diagram of conjugating CSTSMLKAC to exosomes by a two-step reaction. (B) Transmission electron micrograph of Exo^Scr and Exo^CTP. Scale bar, 50 nm. (C, D) Atomic force microscopy images of Exo^Scr and Exo^CTP. Scale bar, 200 nm. (E) Particle size distribution determined by nanoparticle tracking analysis (NTA). (F, G) Zeta potential and polydispersity index (PDI) of Exo^Scr and Exo^CTP were determined using dynamic light scattering (n = 4). (H, I) Immunocolocation of exosomes was performed by NanoView microarray. The chips were coated with antibodies against anti-mouse CD81 and CD9 individually. To determine the co-localization, fluorescently labeled antibodies against CD9, CD81 and CD63 were employed. Scale bar, 10 μm. All data are represented as mean ± SD. Two-tailed unpaired Student’s t test was utilized. ns: not significant.