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(A and B) IP of FLAG-agarose in SEL1L–/– HEK293T cells transfected with G585D or M528R SEL1L-FLAG to test their interactions with components of the ERAD complex, with quantitation shown in B. n = 3–4 per group. n, individual cell samples. Data are represented as means ± SEM. ***P < 0.001; ****P < 0.0001, 1-way ANOVA followed by Dunnett’s multiple-comparisons test (B).
