Figure 6. DNA methylation contributes to the maintenance of GD hematopoietic memory in adult.

(A) Immunoblot analysis of DNMT1 and DNMT3A in LSK cells isolated from Ctrl, GD offspring, (WT^Akita and WT^STZ), and adult mice with full-blown diabetes triggered by the Ins2^Akita mutation or STZ treatment. (B) Schematic of experimental design for in vivo treatment with vehicle or 5-azadC. (C) BM cellularity in WT^STZ offspring directly after 4 weeks of 5-azadC treatment (n = 5/group) or a 2-week recovery period after treatment (n = 4/group). (D) Immunoblot analysis of DNMT1 and DNMT3A in c-Kit⁺ cells isolated from WT^STZ after 5-azadC treatment or recovery period. (E) Absolute numbers of BMDMs in culture 24 hours after treatment with PBS or LPS/IFN-γ for 5-azadC–treated WT^STZ offspring after treatment (n = 12) or recovery period (n = 8). Data are represented as means ± SD. Unpaired 2-tailed Student’s t tests (C) and 2-way ANOVA with Šidák’s post hoc test (E). *P ≤ 0.05; ***P ≤ 0.0005.