Figure 5. UPFL1 cell lines are sensitive to FGFR inhibition with erdafitinib.

(A) Representative bright-field and GFP images of an epithelial island surrounded by GFP-expressing irradiated fibroblasts. (B) PCR demonstrating evidence of LoxP recombination of the LSL-FGFR3 S249C allele in UPFL1 and UPFL3 cell lines, while liver tissue shows only the targeted allele. (C) IC₅₀ curves were generated in biologic triplicate for UPFL1 and UPFL3 cells’ response to erdafitinib. UPPL1541 cells serve as a control for non–FGFR3-mutated murine bladder cancer cell line. (D) Immunoblots with the indicated antibodies in whole-cell extracts of UPFL1 cells treated with erdafitinib for the indicated dose and time.