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. 2023 Dec 4;56(1):37–50. doi: 10.1038/s41588-023-01600-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Examples of blood cell distributions in the WDF channel for three technical replicates of four randomly selected donors under baseline and Pam3CSK4 19 h conditions. Replicates were performed on samples collected from the same blood draw. b, Examples of blood cell distributions in the WDF channel for three longitudinal replicates of four randomly selected donors under baseline and Pam3CSK4 19 h conditions. Replicates were performed on the same donor from samples collected at different time points that are months apart. c, Examples of three blood traits calculated from our flow cytometry gates (NE2/NE4 ratio, RBC1 Med SSC, and PLT-F CV SFL) under baseline and Pam3CSK4 19 h conditions for data collected over the course of four months. The black dots shown indicate all study participants. The time-dependent trajectories of four donors (same individuals as shown in b) were plotted in colors. Boxplots for daily measurements represent the interquartile range (IQR) between the first and third quartiles as the box, the median as the line inside the box, and the whiskers extend from the box to the largest and smallest values within 1.5× IQR, with any points outside of this range shown as individual outliers.