Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 4;56(1):100–111. doi: 10.1038/s41588-023-01603-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, Heatmaps (bottom) and average plots (top) comparing chromatin accessibility assayed by S5P CUTAC, with RNAPII-S5P and BRG1 occupancy (CUT&Tag), relative to the primary peaks (summits) of S5P CUTAC and sorted by decreasing accessibility (CUTAC signal). b, Schematic showing distinct stages in RNAPII transcription that are inhibited by drugs used in this study. c,e,g, Violin plots of spike-in calibrated CUT&Tag signal distribution comparing RNAPII-S5P and BRG1 occupancy over S5P CUTAC peaks at time points after drug treatments for transcription inhibition: Triptolide (c), Flavopiridol (e) and Actinomycin D (g). Median values (solid lines), upper and lower quartiles (broken lines) and outliers were calculated using the Tukey method; n = 9,700. d,f,h, Fold changes in mean RNAPII-S5P and BRG1 occupancy (spike-in calibrated CUT&Tag) over S5P CUTAC peaks at time points after drug treatments: Triptolide (d), Flavopiridol (f) and Actinomycin D (h). All datasets are representative of at least two biological replicates. The RNAPII illustration was created with BioRender.com. Nuc, nucleosome; PIC, pre-initiation complex.