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. 2023 Dec 4;56(1):100–111. doi: 10.1038/s41588-023-01603-8

Fig. 2. Enrichment of BAF increases nucleosome eviction.

Fig. 2

a, Comparison of chromatin structure and chromatin accessibility by means of S5P CUTAC fragment size distribution over peaks (promoter and enhancer NDR spaces) in cells treated with DMSO (control) and Flavopiridol. Peaks were called with DMSO control. b, Heatmaps comparing nucleosomal (≥150-bp reads) and subnucleosomal (≤120-bp reads) protection by BAF (BRG1 CUT&RUN) and BAF-associated histones (BRG1 CUT&RUN.ChIP) in untreated (DMSO control) cells. Heatmaps were plotted relative to S5P CUTAC summits and sorted by decreasing accessibility (CUTAC signal). CUT&RUN.ChIP heatmaps show enrichment over IgG isotype control (for ChIP). c,d, Enrichment of nucleosomal (≥150-bp, solid lines) and subnucleosomal (≤120-bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative to S5P CUTAC summits, in DMSO- (c) or Flavopiridol-treated (d) cells. e, Flavopiridol treatment causes eviction of partially unwrapped nucleosomes through enrichment of BAF, leading to NDR persistence. All datasets are representative of at least two biological replicates. DMSO, dimethylsulfoxide.