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. 2023 Dec 4;56(1):100–111. doi: 10.1038/s41588-023-01603-8

Fig. 3. Nucleosome eviction by BAF is ATP-dependent.

Fig. 3

a,b, Heatmaps (bottom) and average plots (top) comparing BRG1 occupancy by CUT&RUN upon 8 h of BRM014 treatment versus DMSO control (a), and dual inhibition with BRM014 plus Flavopiridol versus Flavopiridol only (b). Data are plotted relative to the primary peaks (summits) of S5P CUTAC and sorted by decreasing BRG1 CUT&RUN in DMSO. c,d, Enrichment of nucleosomal (≥150-bp, solid lines) and subnucleosomal (≤120-bp, broken lines) reads from BRG1 CUT&RUN.ChIP experiments relative to S5P CUTAC summits, in BRM014 over DMSO control (c) and upon dual inhibition with Flavopiridol and BRM014 over Flavopiridol only (d). Normalized counts of reads in DMSO were subtracted from BRM014 (c), and normalized counts of reads in Flavopiridol only were subtracted from the dual inhibitor treatment (d). All datasets are representative of two biological replicates. FLV, Flavopiridol.