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. 2023 Dec 4;56(1):100–111. doi: 10.1038/s41588-023-01603-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Heatmaps (bottom) and average plots (top) of RNAPII-S5P CUTAC separated by fragment size, relative to primary peaks (summits) of RNAPII-S5P CUTAC. b, Comparison of RNAPII-S5P CUTAC fragment size distribution over peaks (promoter and enhancer NDR spaces) in cells treated with DMSO (control) and Flavopiridol; same data as used for Fig. 2A, plotted differently. c-f, Enrichment of nucleosomal (≥150 bp, solid lines) and subnucleosomal (≤120 bp, broken lines) reads from BRG1 CUT&RUN and CUT&RUN.ChIP experiments, relative to gene promoter TSSs showing RNAPII-S5P enrichment (c, d) and distal regulatory sites (e, f), in DMSO (c, e) and Flavopiridol (d, f) treated cells. CUT&RUN.ChIP data were plotted as enrichment in histone ChIP over IgG isotype control. All datasets are representative of at least two biological replicates.