FIG. 6.

Restriction map of 6.3 kb of E. chaffeensis genomic DNA including the omp-1 gene copies. The four DNA fragments pPS2.6, pPS3.6, pEC2.6, and pEC3.6 were cloned from the genomic DNA. Recombinant plasmid pPS2.6 has a sequence overlapping that of pEC3.6. The black boxes at the bottom show PCR-amplified fragments from the genomic DNA for confirmation of the overlapping area. Open boxes at the top indicate ORFs of omp-1 gene copies, with directions indicated by arrows. Open boxes at the bottom show DNA fragments subcloned for DNA sequencing.