Fig. 2. Production of asymmetric fungal FAS.

A schematic representation for biosynthesis of asymmetric FAS proteins (i.e., prep 1). The schematics of a yeast cells expressing asymmetric FAS protein is shown. The promoters and terminators of each gene are shown as a color-coded line flanking the respective gene. B SDS-PAGE analysis of purified FAS from S. cerevisiae yeast alone or transformed with vector expressing the β-α fusion polypeptide. A representative negative stain micrograph of FAS proteins purified from yeast transformed with the fusion FAS vector.