Fig. 4. In silico purification and alignment of FAS complexes containing one MBP tag.

Polypeptides composing each sample in panels (A) from prep 4 and (B) prep 5 are shown on the top. Transparent cryoEM map in the middle, shows the region of the complex used in focused 3D classification. 2D projections of selected 3D cryoEM density maps are shown. All 3D maps and 2D projections are shown from the same viewing direction.