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. 2024 Jan 12;15:478. doi: 10.1038/s41467-023-42762-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Cycling cells (Fig. 1c) were scored based on gene signatures of all other cell types in the VS TME (e.g., nmSC, T cells, etc.) and assigned to the cell type for which they scored highest. Frequencies of each cell type observed in this cluster were compared to expected rates. b Violin plots of G2M and S-phase scores for Schwann, stromal, and immune cells. c Double-stain IHC of representative high-power field (HPF) from VS tumor FFPE samples. Cycling cells are labeled Ki67 and immune cells are labeled with CD45. Arrowhead indicates a representative CD45-Ki67+ cell. Arrows indicate representative CD45 + Ki67+ cells (scale bar = 50 μm). d Barplot showing the fraction of CD45+ (red) and CD45- (green) cells that are Ki67+ within available samples (left) and averaged across all samples (right). Error bars on left show standard error for quantification of each group across 3–6 HPF. Error bars on the right represent standard error of mean measurements across samples (n = 9 samples). Two-sided t-test was used for comparison. Source data are provided as a Source Data file.