TABLE 2.

Competitive effects of the binding of yeasts treated with amino acids to immobilized substrates

Substrate	% bindinga
	Alanine	Proline	Leucine	Lysine	Arginine
Alanine	0.52*	101.2	99.0	0.4*	98.8
Proline	105.6	0*	0.2*	0.6*	105.6
Leucine	2.70*	95.8	3.9*	92.1	67.5**
Lysine	97.8	106.8	110.1	0.07*	0.2*
Arginine	76.2**	86.2	88.8	2.5*	1.5*
BSA	45.6***	76.2**	67.4**	94.5	91.7

^aYeast cells were pretreated with 1 mg of appropriate amino acid per ml. Thereafter, each immobilized substrate was challenged with pretreated yeasts. The values represent the mean percent binding (n = 12) of yeasts to each immobilized substrate from a typical experiment. Standard deviations were always <10%. *, value significantly different at P < 0.001 compared with the binding of untreated yeasts to the corresponding substrate; **, value significantly different at P < 0.05 compared with the binding of untreated yeasts to the corresponding substrate; ***, value significantly different at P < 0.01 compared with the binding of untreated yeasts to the corresponding substrate.