Fig. 3. Itch is found at the death-inducing signalling complex (DISC) but does not regulate its composition or cleavage of caspase-8.

A Immunoprecipitation of the DISC using an activating TRAIL-R2 antibody from OE33 cells with a stable Itch knockdown (KD) or a control. Cells were treated for 1 h to induce formation of the DISC. Proteins were separated by size on a SDS-PAGE gel prior to Western blot analysis. B Densitometry of cleaved caspase-8 normalised to the pro-caspase-8 in the DISC IP from OE33 cells from three independent experiments. C Immunoprecipitation of the DISC in HT29 cells treated with control or ITCH siRNA using the TRAIL-R2 antibody. D Immunoprecipitation of the DISC in HCT116 cells treated with control or ITCH siRNA using the TRAIL-R2 antibody. Statistical significance was measured by Student’s t test, ns represents a non-significant difference.