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. 2024 Jan 12;15(1):40. doi: 10.1038/s41419-023-06417-4

Fig. 6. Itch regulates intrinsic apoptosis, Bax activation and cytochrome release.

Fig. 6

A Western blot analysis of an immunoprecipitation experiment using the Bax 6A7 antibody that specifically recognises the activated form of the protein. Total Bax and actin from the OE33 detergent extracts are shown below. Cells were treated with 50 ng/ml izTRAIL for 6 h. B Quantification of three independent experiments shown in (A). Statistical analysis was performed using Student’s t test. **p < 0.01. C Flow analysis of cytochrome c release from OE33 control and Itch KD cell lines following stimulation with 10–20 ng/ml izTRAIL for 6 h. In total, 10,000 cells per sample were analysed in three independent experiments. Statistical analysis was performed using 2-way ANOVA. ***p < 0.001. D The percentage apoptotic cells were assessed post-treatment with 5 µM Navitoclax (ABT-263) at the indicated time points. Statistical analysis was performed using 2-way ANOVA. ***p < 0.001.