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. 2024 Jan 12;15(1):36. doi: 10.1038/s41419-023-06414-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Schematic diagram of experimental design. B Immunoblot of microglial NLRP3 expression in WT and ${NLRP 3}_{cxc 3 r 1}^{- / -}$ mice four weeks after CMI induction, n = 12 mice per group. C Immunoblot of microglial H3K4me1/3 expression in WT and ${NLRP 3}_{cxc 3 r 1}^{- / -}$ mice four weeks after CMI induction, n = 12 mice per group. Quantitative analysis of H3K4me3 ChIP-qPCR on TNF-α (D), IL-6 (E), IL-1β (F), and IL-10 (G) promoters among contralateral microglia four weeks after CMI induction in WT and ${NLRP 3}_{cxc 3 r 1}^{- / -}$ mice, n = 12 mice per group. Two-way ANOVA, for interaction: p = 0.0110. So, t-tests were used to detect the difference between groups. CMI vs. CMI+ ${NLRP 3}_{cxc 3 r 1}^{- / -}$ : p = 0.0053 (D); For interaction: p = 0.7059. CMI vs. CMI+ ${NLRP 3}_{cxc 3 r 1}^{- / -}$ : p = 0.9965 (E); For interaction: p = 0.0106. So, t-tests were used to detect the difference between groups. CMI vs. CMI+ ${NLRP 3}_{cxc 3 r 1}^{- / -}$ : p = 0.0341 (F); For interaction: p = 0.0003. So, t-tests were used to detect the difference between groups. CMI vs. CMI+ ${NLRP 3}_{cxc 3 r 1}^{- / -}$ : p = 0.6246 (G) with Tukey’s correction. Representative Nissl and Iba1 IHC staining (H) and quantitative analysis of infarct size and microglial activation (I–L) showing that NLRP3 blockade in microglia after CMI abolished the exacerbated infarct size and microglial activation by CMI, n = 12 mice per group. Two-way ANOVA, for interaction: p = 0.6293. CMI vs. CMI+ ${NLRP 3}_{cxc 3 r 1}^{- / -}$ : p = 0.0231 (I), for interaction: p = 0.2277. CMI vs CMI+ ${NLRP 3}_{cxc 3 r 1}^{- / -}$ : p = 0.0034 (K), for interaction: p = 0.0617. CMI vs. CMI+ ${NLRP 3}_{cxc 3 r 1}^{- / -}$ : p = 0.0229 (L) with Tukey’s correction. Data are presented as mean ± standard deviation (SD), *p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant.