Fig. 1.

Increasing HbA1c correlated with changes to CD4⁺ T cell subset populations. A Gating strategy employed to characterize CD8⁺ and CD4⁺ T cell populations. Fluorochrome-labeled antibodies were used to characterize the T cell populations. PBMCs were stained for lymphocyte and differentiation markers as outlined in the methods. B Correlation analysis between % HbA1c and CD8⁺ TCentral Memory (Tcm), TNaïve, TEffector Memory (T_EM) and T terminally differentiated effector memory (T_ERMA) subsets, overall CD8⁺ T cell population, and CD4:CD8 ratio. Data points represent individual donors (n = 88). Statistical significance was determined using simple linear regression, with significant P values displayed. C Correlation analysis between % HbA1c and CD4⁺ TCentral Memory, TNaïve, TEffector Memory and T terminally differentiated effector memory (TERMA), and overall CD4⁺ T cell population. Data points represent individual donors (n = 88). Statistical significance was determined using simple linear regression, with significant P values displayed. B, C Donors without diabetes are represented by green triangles. Donors with diabetes are represented by blue circles