Fig. 2.

Percentage of CD8⁺ T cells expressing TNF-α following IAV infection inversely correlates to HbA1c. A Gating strategy employed to investigate CD8⁺ and CD4⁺ T cell response to stimulation. Fluorochrome-labeled antibodies were used to identify the CD4⁺ and CD8⁺ T cell populations expressing IFN-γ, TNF-α, MIP1-β and CD107a. B Correlation analysis between % HbA1c and frequency of cells expressing IFN-γ, TNF-α, CD107a and MIP-1β, with background staining subtracted. CD8⁺ T cells were infected with IAV (HKx31, H3N2) for 18 h. Results are relative to negative control condition (unstimulated cells). Data points represent individual donors (n = 88). Statistical significance was determined using simple linear regression, with significant P values displayed. A, B Donors without diabetes are represented by green triangles. Donors with diabetes are represented by blue circles