Figure 1.

mRNAs are frequentlym⁵C-hypermethylated in HCC tissuesA. Distribution pattern of the m⁵C sites on mRNAs in the HCC tissues (tumor) and the adjacent tissues (normal). B. Different proportions of m⁵C modifications in regions of mRNA between the HCC tissues and the adjacent tissues. C. The overall m⁵C modification level is higher in the HCC tissues than in the adjacent tissues, as determined by the BisSeq data analysis. Statistical significance was calculated by Wilcoxon test (****, P = 5.116E−09). D. Difference in the m⁵C modification levels between the HCC tissues and the adjacent tissues. E. Heatmap showing the differential m⁵C methylation levels between the HCC tissues and the adjacent tissues. HCC, hepatocellular carcinoma; m⁵C, 5-methylcytidine; CDS, coding sequence; UTR, untranslated region.