Supplementary Figure S1.
Distribution characteristics of m5C in HCC and expression pattern of target genes. A. The type and proportion of RNA in the sequencing library. B. The sequences are proximal to the mRNA m5C sites in HCC tissues and the adjacent tissues. C. The m5C hypermethylated and highly expressed genes in HCC tissues were involved in different cellular processes, determined by the GO functional analysis. D. and E. The expression of target genes, including GRB2, AATF, RNF115, ADAM15, RTN3, and HDGF, was higher in HCC tissues than that in the adjacent tissues, determined by transcriptome analysis, ****, P < 0.0001. F. The overall survival analysis showed that the higher expression of ADAM15, RTN3, and HDGF was correlated with a poor prognosis in HCC patients; ADAM15 (*, P = 0.0097), RTN3 (**, P = 0.00018), HDGF (*, P = 0.0092). TPM, transcripts per kilobase of exon model per million mapped reads; HCC, hepatocellular carcinoma; GO, gene ontology.